Curation Information

Dual regulation of genes involved in acetoin biosynthesis and motility/biofilm formation by the virulence activator AphA and the acetate-responsive LysR-type regulator AlsR in Vibrio cholerae.;Kovacikova G, Lin W, Skorupski K;Molecular microbiology 2005 Jul; 57(2):420-33 [15978075]
AphA [H9L4T0, view regulon]
Reported TF sp.
Vibrio cholerae O1 biovar El Tor str. N16961
Reported site sp.
Vibrio cholerae O1 biovar El Tor str. N16961
Created by
Elliot White
Curation notes

Experimental Process

Repression of the acetoin biosynthesis pathway by AphA was initially indicated by a DNA-array expression analysis, and later by lacZ-promoter fusion assay. Researchers used an EMSA of the VC1589 promoter region to demonstrate binding.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... VC1588 VC1589 VC1590 VC1591
Experimental technique details Beta-gal reporter assay - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Western blot (quantitative) expression analysis (ECO:0000279) - repressor dimer