Curation Information

ohrR and ohr are the primary sensor/regulator and protective genes against organic hydroperoxide stress in Agrobacterium tumefaciens.;Chuchue T, Tanboon W, Prapagdee B, Dubbs JM, Vattanaviboon P, Mongkolsuk S;Journal of bacteriology 2006 Feb; 188(3):842-51 [16428387]
OhrR [A9CJU2, view regulon]
Reported TF sp.
Agrobacterium tumefaciens NTL4
Reported site sp.
Agrobacterium tumefaciens NTL4
Created by
Dinara Sagitova
Curation notes

Experimental Process

lacZ transcriptional fusion analysis showed that OhrR acts as a repressor. EMSAs showed the direct interaction of OhrR with the ohr and ohrR promoters. DNase I footprinting was used for precise localization of the OhrR binding site within the ohr-ohrR intergenic region.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... ohrR ohr Atu0845 Atu0844 Atu0848 Atu0849 Atu0850
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - repressor dimer