Curation Information

Publication
Genetic and computational identification of a conserved bacterial metabolic module.;Boutte CC, Srinivasan BS, Flannick JA, Novak AF, Martens AT, Batzoglou S, Viollier PH, Crosson S;PLoS genetics 2008 Dec; 4(12):e1000310 [19096521]
TF
IolR [Q9A8Q6, view regulon]
Reported TF sp.
Caulobacter crescentus CB15
Reported site sp.
Caulobacter crescentus CB15
Created by
Erill Lab
Curation notes
-
External databases
NCBI GEO (GSE12414) http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12414.

Experimental Process

Genes involved in myo-Inositol catabolism identified by transposon mutagenesis and DNA-array assays. Represssion of target genes shown by beta-gal assays with iolR mutant. Putative motif identified by MEME, then verified with beta-gal with site-directed mutagenesis.

Transcription Factor Binding Sites


GGACCATATGTTCCA
GGACCATATGTTCCA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
GGACCATATGTTCCA CC_1298,
... ... CC_1298 CC_1297 CC_1299 CC_1300 CC_1301 CC_1302 CC_1303 CC_1304 CC_1305
Experimental technique details Ad-hoc qualitative phenotype observation (ECO:0005674) - Experimental technique details Beta-gal reporter assay - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Random mutagenesis (ECO:0005530) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor not specified