RT-PCR analysis was done to verify that CodY regulates tcdR gene expression. EMSA showed that CodY directly interacts with its target promoter. DNase I footprinting assay was used to identify the specific binding sites for CodY.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|