Curation Information

Repression of Clostridium difficile toxin gene expression by CodY.;Dineen SS, Villapakkam AC, Nordman JT, Sonenshein AL;Molecular microbiology 2007 Oct; 66(1):206-19 [17725558]
CodY [Q18BE1, view regulon]
Reported TF sp.
Clostridium difficile 630
Reported site sp.
Clostridium difficile 630
Created by
Dinara Sagitova
Curation notes

Experimental Process

RT-PCR analysis was done to verify that CodY regulates tcdR gene expression. EMSA showed that CodY directly interacts with its target promoter. DNase I footprinting assay was used to identify the specific binding sites for CodY.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type