CodY-regulated genes were identified by DNA microarray analysis. qRT-PCR analyses were performed to confirm the gene expression results obtained from the microarray analysis. EMSAs were done to validate that CodY directly interacts with the promoters of the identified genes. DNase I footprinting was used to identify specific binding sites for CodY.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|