EMSA was performed to validate binding of purified LexA upstream of the lexA promoter. DNAse footprint was used to specify site, mutagenesis to precisely define it. RT-PCR was used to confirm expression induced with mitomycin C
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|