Curation Information

Publication
Activation of both acfA and acfD transcription by Vibrio cholerae ToxT requires binding to two centrally located DNA sites in an inverted repeat conformation.;Withey JH, DiRita VJ;Molecular microbiology 2005 May; 56(4):1062-77 [15853890]
TF
ToxT [A5F384, view regulon]
Reported TF sp.
Vibrio cholerae O395
Reported site sp.
Vibrio cholerae O395
Created by
Erill Lab
Curation notes
-

Experimental Process

Combination of beta-gal assays with limited site-directed mutagenesis on the shared acfD-acdA promoter. Verification of binding sites through copper-phenanthroline footprinting

Transcription Factor Binding Sites


AATTTTTAAAAAT
CATTTTGTTAAAT
AATTTTTAAAAAT
CATTTTGTTAAAT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
AATTTTTAAAAAT acfA, VC0395_A0369,
... ... acfA VC0395_A0369 tagE-1 VC0395_A0370
Experimental technique details Beta-gal reporter assay - Experimental technique details Copper-phenanthroline footprinting (ECO:0005626) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator monomer
CATTTTGTTAAAT acfA, VC0395_A0369,
... ... acfA VC0395_A0369 tagE-1 VC0395_A0370
Experimental technique details Beta-gal reporter assay - Experimental technique details Copper-phenanthroline footprinting (ECO:0005626) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator monomer