Curation Information

Fur functions as an activator and as a repressor of putative virulence genes in Neisseria meningitidis.;Delany I, Rappuoli R, Scarlato V;Molecular microbiology 2004 May; 52(4):1081-90 [15130126]
Fur [P0A0S8, view regulon]
Reported TF sp.
Neisseria meningitidis MC58
Reported site sp.
Neisseria meningitidis MC58
Created by
Dinara Sagitova
Curation notes

Experimental Process

Putative Fur binding sites were identified by screening N. meningitidis MC58 genome with Fur-box sequence of E. coli. DNase I footprinting was used to demonstrate binding of Fur to the predicted consensus binding sites. S1 nuclease protection assay showed Fur represses tbp promoter in response to high iron.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... tbp2 NMB0459 tbp1
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details PSSM site search (ECO:0005659) - Experimental technique details S1 nuclease protection (ECO:0005666) - repressor not specified