Curation Information

Publication
Identification of AlgR-regulated genes in Pseudomonas aeruginosa by use of microarray analysis.;Lizewski SE, Schurr JR, Jackson DW, Frisk A, Carterson AJ, Schurr MJ;Journal of bacteriology 2004 Sep; 186(17):5672-84 [15317771]
TF
AlgR [P26275, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Erill Lab
Curation notes
-

Experimental Process

Differentially expressed genes identified by DNA-array. Putative sites identified by MSA with known (algD) promoter sites. AlgR dependent expression confirmed by primer extension.

Transcription Factor Binding Sites


CCGGGCGTC
CCATGCTTC
CCGGGCGTC
CCATGCTTC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CCGGGCGTC ccoN2, ccoO2, ccoQ2, ccoP2,
... ... ccoN2 ccoO2 ccoQ2 ccoP2 PA1558 PA1559 PA1560 aer
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details Primer Extension assay (ECO:0005657) - repressor dimer
CCATGCTTC ccoN2, ccoO2, ccoQ2, ccoP2,
... ... ccoN2 ccoO2 ccoQ2 ccoP2 PA1558 PA1559 PA1560 aer
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details Primer Extension assay (ECO:0005657) - repressor dimer