Site identified by EMSA and DNAse footprint with crude extract on gltA promoter. Site has nice dyad symmetry. Site function verified by beta-gal assays coupled with directed mutagenesis. Protein identified by systematic EMSA of putative repressors encoded in G. sulfurreducens on the gltA promoter.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|