Sites were identified by fusing different length promoter fragments with a promoterless LacZ and comparing B-gal activity. The results of these assays suggest the presence of 3 sites in the ompU promoter. The existance of these sites was verified with emsas of the promoter fragments, and a DNase protection assay, which showed three footprints. The authors do not attempt to narrow down the sites further.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|