Curation Information

Analysis of ToxR-dependent transcription activation of ompU, the gene encoding a major envelope protein in Vibrio cholerae.;Crawford JA, Kaper JB, DiRita VJ;Molecular microbiology 1998 Jul; 29(1):235-46 [9701817]
ToxR [A0A0H3AK48, view regulon]
Reported TF sp.
Vibrio cholerae O395
Reported site sp.
Vibrio cholerae O395
Created by
Elliot White
Curation notes

Experimental Process

Sites were identified by fusing different length promoter fragments with a promoterless LacZ and comparing B-gal activity. The results of these assays suggest the presence of 3 sites in the ompU promoter. The existance of these sites was verified with emsas of the promoter fragments, and a DNase protection assay, which showed three footprints. The authors do not attempt to narrow down the sites further.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type