Using a Dnase protection assay, researchers determined the presence of three CodY-protected sites around the transcription start. The one upstream site was seen to only be bound at high concentrations. Researchers then preformed EMSA's and B-gal assays on the two downstream sites, which showed that the sites regulated independantly, and that the first site was tighter bound, and responsible for most of the regulatory effect. Site directed mutagenesis was then used to confirm the role of site 1, and quantify the regulatory effect of site 2.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|