Curation Information

Publication
Regulation of haemolysin (VvhA) production by ferric uptake regulator (Fur) in Vibrio vulnificus: repression of vvhA transcription by Fur and proteolysis of VvhA by Fur-repressive exoproteases.;Lee HJ, Kim JA, Lee MA, Park SJ, Lee KH;Molecular microbiology 2013 May; 88(4):813-26 [23560801]
TF
Fur [P33117, view regulon]
Reported TF sp.
Vibrio vulnificus MO6-24/O
Reported site sp.
Vibrio vulnificus MO6-24/O
Created by
Erill Lab
Curation notes
-

Experimental Process

Site in vvhBA promoter identified by DNAse footprinting. Binding further confirmed by site-directed mutagenesis and EMSA. Evidence of regulation with lux fusion assay. Effect on haemolysin production quantified. Evidence of regulation by western of VvhA

Transcription Factor Binding Sites


TATTTTCAGGATTATTAAT
TATTTTCAGGATTATTAAT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TATTTTCAGGATTATTAAT VVMO6_03880, VVMO6_03881,
... ... VVMO6_03880 VVMO6_03881 VVMO6_03879
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Luciferase reporter assay (ECO:0005648) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Western blot (quantitative) expression analysis (ECO:0000279) - repressor dimer