Curation Information

Publication
Recognition of DNA by three ferric uptake regulator (Fur) homologs in Bacillus subtilis.;Fuangthong M, Helmann JD;Journal of bacteriology 2003 Nov; 185(21):6348-57 [14563870]
TF
Fur [P54574, view regulon]
Reported TF sp.
Bacillus subtilis subsp. subtilis str. 168
Reported site sp.
Bacillus subtilis subsp. subtilis str. 168
Created by
Erill Lab
Curation notes
-

Experimental Process

Binding shown with DNAse footprinting. Regulation demonstrated with beta-gal assays on fur mutant.

Transcription Factor Binding Sites


TGATAATAGTTATCA
TGATAATTATTATCA
TGAAGATGATTTACG
TGATAATAGTTATCA
TGATAATTATTATCA
TGAAGATGATTTACG

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TGATAATAGTTATCA feuA,
... ... feuA feuB feuC ybbA ybaS ybaR ybbB
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - repressor dimer
TGATAATTATTATCA zosA,
... ... zosA stoA ykvU ykvT
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - repressor dimer
TGAAGATGATTTACG zosA,
... ... zosA stoA ykvU ykvT
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - repressor dimer