Curation Information

Recognition of DNA by three ferric uptake regulator (Fur) homologs in Bacillus subtilis.;Fuangthong M, Helmann JD;Journal of bacteriology 2003 Nov; 185(21):6348-57 [14563870]
PerR [P71086, view regulon]
Reported TF sp.
Bacillus subtilis subsp. subtilis str. 168
Reported site sp.
Bacillus subtilis subsp. subtilis str. 168
Created by
Erill Lab
Curation notes

Experimental Process

DNAse I footprinting and beta-gal assays with perR mutant were used to demonstrate binding and repression of PerR to the mrgA promoter, in combination with site-directed mutagenesis.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... mrgA yusZ yusY yusY yusW htrB
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor dimer