Proteoase activity assay determines iron regulation of vvpE. Repression by Fur shown with luciferase assays with fur mutant. Two binding regions identified by DNAse footprinting. Site-directed mutagenesis with EMSA and luciferase assays to demonstrate importance of both binding regions. Sites identified based on similarity to described consensus.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|