Curation Information

Mutation analysis of the Pseudomonas aeruginosa mvfR and pqsABCDE gene promoters demonstrates complex quorum-sensing circuitry.;Xiao G, He J, Rahme LG;Microbiology (Reading, England) 2006 Jun; 152(Pt 6):1679-86 [16735731]
LasR [P54292, view regulon]
Reported TF sp.
Pseudomonas aeruginosa UCBPP-PA14
Reported site sp.
Pseudomonas aeruginosa UCBPP-PA14
Created by
Erill Lab
Curation notes

Experimental Process

A previously reported site was shown to be necessary to activate pqsA transcription using beta-gal assays coupled to site-directed mutagenesis.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... pqsA pqsB pqsC pqsD pqsE phnA phnB mvfR ogt cupC3 cupC2 cupC1
Experimental technique details Beta-gal reporter assay - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - repressor not specified