Curation Information

Publication
The FleQ protein from Pseudomonas aeruginosa functions as both a repressor and an activator to control gene expression from the pel operon promoter in response to c-di-GMP.;Baraquet C, Murakami K, Parsek MR, Harwood CS;Nucleic acids research 2012 Aug; 40(15):7207-18 [22581773]
TF
FleQ [G3XCV0, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Erill Lab
Curation notes
-

Experimental Process

DNAse footprinting and a site directed mutagenesis assay were used to locate two binding sites. A beta-gal assay was used to confirm expression.

The paper reports that TF forms complex with other proteins for binding with reported sites.

FleQ is bound to FleN, which allows activation in the presence of c-di-GMP. In its absence, FleQ operates as a repressor.

Transcription Factor Binding Sites


CGCCTAAAAATTGACAGTT
CATTAGATTGACGTTAATC
CGCCTAAAAATTGACAGTT
CATTAGATTGACGTTAATC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CGCCTAAAAATTGACAGTT pelA,
... ... pelA pelB pelC pelD pelE pelF pelG PA3057 PA3056 PA3055 PA3054 PA3065 PA3066 PA3067 gdhB
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor not specified
CATTAGATTGACGTTAATC pelA,
... ... pelA pelB pelC pelD pelE pelF pelG PA3057 PA3056 PA3055 PA3054 PA3065 PA3066 PA3067 gdhB
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor not specified