Curation Information

Autogenous transcriptional regulation of the regA gene, encoding an AraC-Like, essential virulence regulator in Citrobacter rodentium.;Tan A, Yang J, Tauschek M, Praszkier J, Robins-Browne RM;Journal of bacteriology 2011 Apr; 193(7):1777-82 [21278290]
RegA [D2TIS0, view regulon]
Reported TF sp.
Citrobacter rodentium ICC168
Reported site sp.
Citrobacter rodentium ICC168
Created by
Erill Lab
Curation notes

Experimental Process

EMSA determines binding. Betal-gal assay with RegA mutant proves regulation. Site identified by comparison to Rns consensus. Binding further verified by DNAse footprint. Binding site corroborated by site-directed mutagenesis coupled to beta-gal.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... ROD_41311 adcA
Experimental technique details Beta-gal reporter assay - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator not specified