Curation Information

SOS induction in mycobacteria: analysis of the DNA-binding activity of a LexA-like repressor and its role in DNA damage induction of the recA gene from Mycobacterium smegmatis.;Durbach SI, Andersen SJ, Mizrahi V;Molecular microbiology 1997 Nov; 26(4):643-53 [9427395]
LexA [A0QVY5, view regulon]
Reported TF sp.
Mycobacterium smegmatis str. MC2 155
Reported site sp.
Mycobacterium smegmatis str. MC2 155
Created by
Erill Lab
Curation notes

Experimental Process

Binding demonstrated with EMSA, comparison to previously identified motif and site-directed mutagenesis. Induction with mit-C shown with xylE reporter assay.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... recA MSMEG_2737 MSMEG_2736 dapF miaA MSMEG_2733 MSMEG_2732 MSMEG_2731 MSMEG_2730 miaB MSMEG_2728 MSMEG_2727 MSMEG_2726 MSMEG_2725 recX MSMEG_2704 hypE gmhA MSMEG_2707 MSMEG_2709 MSMEG_2708 MSMEG_2710 hypF hypC MSMEG_2713 MSMEG_2714 MSMEG_2715 MSMEG_2716 MSMEG_2717 MSMEG_2718 MSMEG_2719 MSMEG_2720 hypB hypA MSMEG_2738
Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - Experimental technique details xylE reporter assay - repressor dimer