Curation Information

Publication: Global analysis of the regulon of the transcriptional repressor LexA, a key component of SOS response in Mycobacterium tuberculosis.;Smollett KL, Smith KM, Kahramanoglou C, Arnvig KB, Buxton RS, Davis EO;The Journal of biological chemistry 2012 Jun 22; 287(26):22004-14 [22528497]
TF: LexA [I6Y1J7, view regulon]
Reported TF sp.: Mycobacterium tuberculosis H37Rv
Reported site sp.: Mycobacterium tuberculosis H37Rv
Created by: Erill Lab
Curation notes: Submission of peak-values only. Motif-associated sites in separate submission.

Experimental Process

ChIP seq to identify peaks.

ChIP assay conditions: MtuLexA: The mycobacterial strains used were wild-type M. tuberculosis strains H37Rv and 1424 and M. tuberculosis
ChIP notes: Chromatin Immunoprecipitation (ChIP)—ChIP was performed using a method adapted from Sala et al.(16). Mycobacterial cultures were grown to mid-log phase (A600

Transcription Factor Binding Sites

AAGGTGTTCATAGGACATGCCTCCGCCTCCTCACTCGATAGATAGTGAAATGGTTTCCCACTGTTTTGATGTACAGTTAACCCAATTCGAACAAGTGATCGAATCTCGGTCTGCGACACCGAAACCGGCCGGCCAACCGCGAAATGACACTGATGTGATTAGACACAAGTTGGGGACGCGGGTCAAG
GGCGGCCTCCTCGACCAACTCCTCGGGCGTGCTGGCCCCGTCGAGGAAGCTGTACGCCGAATGCGCATGCAGCTCGGCATACGCGACGGACGATCCGACCCGTTCCCGGCCCGGCGGCTGGTACGCCCCGCGCTTGCGGGACCGTGGGACGTCCCCATCCGCGTCGAACGCCGGCACCCCGGCATGGCGCGGCTTGCCGTTAA
GACCGCACCGAGTGCCGTGCTCGCCCTCGGCGGCTACCGAGCCGGTGTCTCCCTAGTCATCCACGTTATCCACAGCGCCTTGGGTTACCGGGCGCCGGTCGGGTAGCGATGGTAGTATCGAAAGTATGTTCGA
CGGTTTGATCAGTAGCGGCAAACCCGGTAGGGACCCCGAGGCTGGGCGCTACGGCGCCCTGCTGAACGCCATTACCGCCGAAATACCACGGGCATCGTCGGGCTGATCTGCCCACGAGCACGCCACGCCGCCCTAACCGAGCCGGACGGCTTTGTCGTGCCGGTCACATGTCGGCCTGT
CAAGACGCGACATCGTAAAGGTTTTAGCCGGACTCATTCGAATATTTGAGCGATGTAGCCAGTGAGTGGGTGCTCCGATGATCACGGCT
TAACATGTTCGAATCATAACGTCGGGCACTGACAAGAAGTCGCGCCGACAGCTGCTAGAACTGGTGTTAGCTAAGTGAATTCAGTGACTCGAGAGCCCTCGCGAGCTTGGCCGCCCACCAGGTCGGCGGGGATGCCTA
CGGCAGCGCCCCGTCGTCAACGGGCATCCCGTTCACCGCGACGGCTTGCCGGGCCCAACGCATACCATTATTCGAACAACCGTTCTATACTTTGTCAACGCTGGCCGCTACCGAGCGCCGCACAGG
GTCGGGCACTGACAAAGAGCGCCCCGCTGATAACCGTGAAACTGAAGTGACACAAGGGATTTACCCAGATCCTACGAGTTGATACGGGAAGG
CATCGGGTCAACCCGGGATCGACACCCATCACCCGCACCGCACGCTCCCTCAGCCATTTCCGAACAATCGTTCGATACGCTAGCGGATCGTCCCGACATCC
ACAGGGGTGCGTTTGTCGGCGGCTGGCGGGGCGTGGCGGCGATAGCATTCGAATATGAGTTCGATCACGGTGTCGGTGGACCCGGTGGACCCGGTGGACCCGGTGG
GGCTTCTCAGACGACCCGTGACGCGAACCGTCGTGCTGTCGGTGGCCGCTACTAGTATCGCACACATGTTCGAGATATCGCTGCCGGACCCGACGGAGCTGTGCCGATCCGATGATGGCGCGCTGGTGGCCG
ATATGCGATTTAGTTGTGTGTAGCTTGTTTTGCTGTCTGTACGACTGCACCGAGGGGTGAGCGCGTGTCGCACGAAAGTCTGTTCGAAGAAAGCGAAGCGCCCTACGCGGCGCTGTGCGTAGTTGC
CCGGACCGGTGTCATGTTCGCTCCTCCAACTCAGACGGTAATCGCTCGCGTGTTCGACACTGTAGTCACTCATGTGTTCGATATCCGAACATTTGATCGA
GCCCCTGGCTGATCCGCAAGGCCGCACCGACCTCAGCGATCACCGCCGCCTGTGACCACTAACCAGTCTCATCGAAAATATATTCGATACAGCCACTTGCCGTCGACATTGACCATGAGGCGTTCACGTCGCAGGGCCG
GCCGAGCTGTCTTCGCTCGTTGTGCTCGAAGAGCGCGCCTTGAACCGCGATCGCCACGCCGCCAAGCTTATCGCACATTCGTTCGATGGCGCCGCCCCGGCTACGGTTTGACCTGTGGGTGTCGAATTGGGGTCAAATTCCGAGGTCGGCGCGCTAAGAGTGGTCATCCTGCACCG
AGACCTTCAGGACCCGGTTAAGGGCGGTGTCGAGGTTGTCGAACGCGGCGACGGCCTCCTCCCGGCTACTCGAATACATGTTCGAATACTATCACGGTTAGCCGGCCGATGCCATGCTGATTGTGGGTTAATCCAATGTGGTGCAGTTGAATTCAGGAGCATCGCCAGCCGCGAGGCCACGCCTATTCGGCGAGCATAATGGTCGG
CGGTGTTCGATTGACCGGTATCGGTCGGTTGGCCGTGACACTAGAGACAGCCACTGACAAGTCGGCTGCTCCGAATGATCACCACAGTAGCCGAACACCTGTTCGATTCAAGTGTGACACGCCGCGTGTGGCAACATCGCGTCCGCGCTCGTCGGCGCGTCGAACGCCCTGGCGGCGGTGCGGCCCGACTTGCG
TATAGCCGTTACCGCGGGGGCTTATCCACAGCCACCGGCGCCACCAGTTGTCCCGGTCTGTGCAGGCTGCTATTCTCGAACACATGTTCGAGACATTGACCGCGATCGACCCGGATGCCGAGGAAGCGGCGTTGATCGAGCGAATCGCCGAGCTGGAGCGGCTTAAGTC
ATTTGACCCGCTCCAGCTCGGCGATCCGCGCGGTCAGGCTCGCCTCATCGTCGCGCGAATCCACGCCCGCGAGGCTCACTAAATCGAACATGTGTTCGAGTATAGCAGGCCTGGGCCACCGCGGCCACCGCACCGCGGGCCCGCAGCGTGCGAGTGCTACGCTGCCGAGCGGTCGACATCCTTTAACGATCCGTCCAGAGA
CCACACCACCGACACGTTTCGGTTGGAGCCGGACTTCCGGCGCGAAGGCCCAGCCACTCCTCGTGCCCTCCCGCGACCGGAACACGCCTGTCGAACACATGTTTGATTCTTGGTGCGAATGCGACTACATTCATTGCCATGAACGACAGCAACGACACCTCGGTTGCCGGCGGAGCCG
TCAGCGCTTTTGGTGCCGAAATGCGGCGAATCCCGGGCAGTCGGCGTCGCACAGCACGGTTGCTGTGCTGTCGCAAGCCTGGAGGCCCGC
GCCGCCGCCAGGGCGATCACGCCGCCGGCCTGCGCGGTGCCCATCACCGTGGTCACGTTGAGCTGAGAGAACACCCGTTCGATAGCCACCACCTCCGGATGATGGGTGTCCAG
GAGCGGGCGGGCAGCAAACGTCTAGCCCACGCAATCCAAAGCCGCTTCGTAACTTTCGCAGAATTGGGCCTTGCTGTGTTAAAGGTCTAGTAGTACAAAGGAACCACGGAAGCCCGGTGA
GCTTGAACCAGCGTCCGCCCGGCGGTTGGTGTCATTGAGCCGTTTGCGTGGATCACTTCCGCTGCTGCTTGATCGGGCCCTGGTCTGTGTCGGCAGCGGCTGGTAGTATCGAAAGTATG
CGGGATCTGGTATCACCGCGAGCCGGCTCAGATCCGCCCCCATCTCCACCGCGGCCAGCAACCCGATATCCGGCTGGCCAACGATGGCCGCGTTTCCCCCGGCCGCCGTCACCGATGCCACCATGCTCAGCAGCAGTGACCGCGCACCCGACAGCACTCCCACCGTCCCCGGGGGCAACGACACCGGTCCCGCCGGCACCAGGTCGCCCGAACGGCTGGGCCCCCCGGACACCTTCTCGGACAGCAAAGCCATCTGCCGTCGTAGTGATTCGAGC

Quantitative data format: ChIP-seq. Posterior enrichment probability . BayesPeak peak calling method.

AAGGTGTTCATAGGACATGCCTCCGCCTCCTCACTCGATAGATAGTGAAATGGTTTCCCACTGTTTTGATGTACAGTTAACCCAATTCGAACAAGTGATCGAATCTCGGTCTGCGACACCGAAACCGGCCGGCCAACCGCGAAATGACACTGATGTGATTAGACACAAGTTGGGGACGCGGGTCAAG 1.0
GGCGGCCTCCTCGACCAACTCCTCGGGCGTGCTGGCCCCGTCGAGGAAGCTGTACGCCGAATGCGCATGCAGCTCGGCATACGCGACGGACGATCCGACCCGTTCCCGGCCCGGCGGCTGGTACGCCCCGCGCTTGCGGGACCGTGGGACGTCCCCATCCGCGTCGAACGCCGGCACCCCGGCATGGCGCGGCTTGCCGTTAA 1.0
GACCGCACCGAGTGCCGTGCTCGCCCTCGGCGGCTACCGAGCCGGTGTCTCCCTAGTCATCCACGTTATCCACAGCGCCTTGGGTTACCGGGCGCCGGTCGGGTAGCGATGGTAGTATCGAAAGTATGTTCGA 1.0
CGGTTTGATCAGTAGCGGCAAACCCGGTAGGGACCCCGAGGCTGGGCGCTACGGCGCCCTGCTGAACGCCATTACCGCCGAAATACCACGGGCATCGTCGGGCTGATCTGCCCACGAGCACGCCACGCCGCCCTAACCGAGCCGGACGGCTTTGTCGTGCCGGTCACATGTCGGCCTGT 1.0
CAAGACGCGACATCGTAAAGGTTTTAGCCGGACTCATTCGAATATTTGAGCGATGTAGCCAGTGAGTGGGTGCTCCGATGATCACGGCT 0.20288
TAACATGTTCGAATCATAACGTCGGGCACTGACAAGAAGTCGCGCCGACAGCTGCTAGAACTGGTGTTAGCTAAGTGAATTCAGTGACTCGAGAGCCCTCGCGAGCTTGGCCGCCCACCAGGTCGGCGGGGATGCCTA 0.13059
CGGCAGCGCCCCGTCGTCAACGGGCATCCCGTTCACCGCGACGGCTTGCCGGGCCCAACGCATACCATTATTCGAACAACCGTTCTATACTTTGTCAACGCTGGCCGCTACCGAGCGCCGCACAGG 0.99754
GTCGGGCACTGACAAAGAGCGCCCCGCTGATAACCGTGAAACTGAAGTGACACAAGGGATTTACCCAGATCCTACGAGTTGATACGGGAAGG 0.35445
CATCGGGTCAACCCGGGATCGACACCCATCACCCGCACCGCACGCTCCCTCAGCCATTTCCGAACAATCGTTCGATACGCTAGCGGATCGTCCCGACATCC 0.99911
ACAGGGGTGCGTTTGTCGGCGGCTGGCGGGGCGTGGCGGCGATAGCATTCGAATATGAGTTCGATCACGGTGTCGGTGGACCCGGTGGACCCGGTGGACCCGGTGG 0.99929
GGCTTCTCAGACGACCCGTGACGCGAACCGTCGTGCTGTCGGTGGCCGCTACTAGTATCGCACACATGTTCGAGATATCGCTGCCGGACCCGACGGAGCTGTGCCGATCCGATGATGGCGCGCTGGTGGCCG 0.99891
ATATGCGATTTAGTTGTGTGTAGCTTGTTTTGCTGTCTGTACGACTGCACCGAGGGGTGAGCGCGTGTCGCACGAAAGTCTGTTCGAAGAAAGCGAAGCGCCCTACGCGGCGCTGTGCGTAGTTGC 0.87757
CCGGACCGGTGTCATGTTCGCTCCTCCAACTCAGACGGTAATCGCTCGCGTGTTCGACACTGTAGTCACTCATGTGTTCGATATCCGAACATTTGATCGA 0.72267
GCCCCTGGCTGATCCGCAAGGCCGCACCGACCTCAGCGATCACCGCCGCCTGTGACCACTAACCAGTCTCATCGAAAATATATTCGATACAGCCACTTGCCGTCGACATTGACCATGAGGCGTTCACGTCGCAGGGCCG 1.0
GCCGAGCTGTCTTCGCTCGTTGTGCTCGAAGAGCGCGCCTTGAACCGCGATCGCCACGCCGCCAAGCTTATCGCACATTCGTTCGATGGCGCCGCCCCGGCTACGGTTTGACCTGTGGGTGTCGAATTGGGGTCAAATTCCGAGGTCGGCGCGCTAAGAGTGGTCATCCTGCACCG 0.99999
AGACCTTCAGGACCCGGTTAAGGGCGGTGTCGAGGTTGTCGAACGCGGCGACGGCCTCCTCCCGGCTACTCGAATACATGTTCGAATACTATCACGGTTAGCCGGCCGATGCCATGCTGATTGTGGGTTAATCCAATGTGGTGCAGTTGAATTCAGGAGCATCGCCAGCCGCGAGGCCACGCCTATTCGGCGAGCATAATGGTCGG 1.0
CGGTGTTCGATTGACCGGTATCGGTCGGTTGGCCGTGACACTAGAGACAGCCACTGACAAGTCGGCTGCTCCGAATGATCACCACAGTAGCCGAACACCTGTTCGATTCAAGTGTGACACGCCGCGTGTGGCAACATCGCGTCCGCGCTCGTCGGCGCGTCGAACGCCCTGGCGGCGGTGCGGCCCGACTTGCG 1.0
TATAGCCGTTACCGCGGGGGCTTATCCACAGCCACCGGCGCCACCAGTTGTCCCGGTCTGTGCAGGCTGCTATTCTCGAACACATGTTCGAGACATTGACCGCGATCGACCCGGATGCCGAGGAAGCGGCGTTGATCGAGCGAATCGCCGAGCTGGAGCGGCTTAAGTC 1.0
ATTTGACCCGCTCCAGCTCGGCGATCCGCGCGGTCAGGCTCGCCTCATCGTCGCGCGAATCCACGCCCGCGAGGCTCACTAAATCGAACATGTGTTCGAGTATAGCAGGCCTGGGCCACCGCGGCCACCGCACCGCGGGCCCGCAGCGTGCGAGTGCTACGCTGCCGAGCGGTCGACATCCTTTAACGATCCGTCCAGAGA 1.0
CCACACCACCGACACGTTTCGGTTGGAGCCGGACTTCCGGCGCGAAGGCCCAGCCACTCCTCGTGCCCTCCCGCGACCGGAACACGCCTGTCGAACACATGTTTGATTCTTGGTGCGAATGCGACTACATTCATTGCCATGAACGACAGCAACGACACCTCGGTTGCCGGCGGAGCCG 1.0
TCAGCGCTTTTGGTGCCGAAATGCGGCGAATCCCGGGCAGTCGGCGTCGCACAGCACGGTTGCTGTGCTGTCGCAAGCCTGGAGGCCCGC 0.73687
GCCGCCGCCAGGGCGATCACGCCGCCGGCCTGCGCGGTGCCCATCACCGTGGTCACGTTGAGCTGAGAGAACACCCGTTCGATAGCCACCACCTCCGGATGATGGGTGTCCAG 0.17685
GAGCGGGCGGGCAGCAAACGTCTAGCCCACGCAATCCAAAGCCGCTTCGTAACTTTCGCAGAATTGGGCCTTGCTGTGTTAAAGGTCTAGTAGTACAAAGGAACCACGGAAGCCCGGTGA 0.99993
GCTTGAACCAGCGTCCGCCCGGCGGTTGGTGTCATTGAGCCGTTTGCGTGGATCACTTCCGCTGCTGCTTGATCGGGCCCTGGTCTGTGTCGGCAGCGGCTGGTAGTATCGAAAGTATG 0.99998
CGGGATCTGGTATCACCGCGAGCCGGCTCAGATCCGCCCCCATCTCCACCGCGGCCAGCAACCCGATATCCGGCTGGCCAACGATGGCCGCGTTTCCCCCGGCCGCCGTCACCGATGCCACCATGCTCAGCAGCAGTGACCGCGCACCCGACAGCACTCCCACCGTCCCCGGGGGCAACGACACCGGTCCCGCCGGCACCAGGTCGCCCGAACGGCTGGGCCCCCCGGACACCTTCTCGGACAGCAAAGCCATCTGCCGTCGTAGTGATTCGAGC 1.0

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence	Regulated genes	Gene diagram	Experimental techniques	TF function	TF type