Curation Information

Fnr, NarP, and NarL regulation of Escherichia coli K-12 napF (periplasmic nitrate reductase) operon transcription in vitro.;Darwin AJ, Ziegelhoffer EC, Kiley PJ, Stewart V;Journal of bacteriology 1998 Aug; 180(16):4192-8 [9696769]
FNR [P0A9E5, view regulon]
Reported TF sp.
Escherichia coli str. K-12 substr. MG1655
Reported site sp.
Escherichia coli str. K-12 substr. MG1655
Created by
Erill Lab
Curation notes

Experimental Process

FNR site identified by DNAse footprinting. Regulation shown by beta-gal assays on fnr mutant and by in vitro transcription (using also NarP and NarL)

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TTGATCCTGCTACA napC, napB, napH, napG, napA, napD, napF,
... ... napC napB napH napG napA napD napF eco ccmH ccmG ccmF ccmE ccmD ccmC ccmB ccmA yojO
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details In-vitro transcription (ECO:0001204) - activator dimer