The location of the CRP that binds between two promoter revealed by DNase I footprinting assay and the in vitro transcription assays showed that CRP activates ideV PS transcription in the presence of cAMP. Electrophoretic mobility shift assays and site directed mutagenesis(EMSAs) used to identify the binding site of CRP in the ideV promoter.
The paper reports that TF forms complex with other proteins for binding with reported sites.
Expression of Vibrio vulnificus insulin-degrading enzyme is regulated by the cAMP–CRP complex.CRP activates ideV PS transcription in the presence of cAMP whose concentration is regulated by EIIAGlc
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|