First a B-gal assay was performed to confirm fur expression. Gel retardation assays were used to demonstrate binding of purified Fur protein to the iron control region. Finally a footprint analysis done using DNAse I, found the TF binding site.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|