Using DNAse I footprinting the Fur specific operator sequences were identified and this also confirmed the binding of Ec-fur to primary and secondary sites. To examine the influence of iron and Fur on the expression of fbpA a gene fusion between the promoter regions of fbpA and chloramphenicol acetyltransferase (CAT) was constructed and PCR amplified to test gene expression.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|