Curation Information

Analysis of Fur binding to operator sequences within the Neisseria gonorrhoeae fbpA promoter.;Desai PJ, Angerer A, Genco CA;Journal of bacteriology 1996 Aug; 178(16):5020-3 [8759870]
Fur [Q5F5Y9, view regulon]
Reported TF sp.
Neisseria gonorrhoeae FA 1090
Reported site sp.
Neisseria gonorrhoeae FA 1090
Created by
Urooq Aqeel
Curation notes

Experimental Process

Using DNAse I footprinting the Fur specific operator sequences were identified and this also confirmed the binding of Ec-fur to primary and secondary sites. To examine the influence of iron and Fur on the expression of fbpA a gene fusion between the promoter regions of fbpA and chloramphenicol acetyltransferase (CAT) was constructed and PCR amplified to test gene expression.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type