Curation Information

Publication
Analyses of the regulatory mechanism and physiological roles of Pseudomonas aeruginosa OhrR, a transcription regulator and a sensor of organic hydroperoxides.;Atichartpongkul S, Fuangthong M, Vattanaviboon P, Mongkolsuk S;Journal of bacteriology 2010 Apr; 192(8):2093-101 [20139188]
TF
OhrR [Q9HZZ4, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Sefa Kilic
Curation notes
OhrR operates as a dimer, but with variable spacer motif. Palindromic monads are captured for curation.

Experimental Process

A beta-gal assay was used to confirm expression, followed by a northern blot. A site-directed mutagenesis, followed by qRT-PCR, was used to show the importance of cysteine residues for expression. EMSA and DNAse footprinting were used to confirm binding of OhrR.

Transcription Factor Binding Sites


ACTTCACGCAC
GCGCAAGAACT
ACTTCACGCAC
GCGCAAGAACT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
ACTTCACGCAC ohr,
... ... ohr PA2852 efp PA2843 PA2844 PA2845 PA2846 PA2847 PA2848 ohrR
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Northern blot (ECO:0005653) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor not specified
GCGCAAGAACT ohr,
... ... ohr PA2852 efp PA2843 PA2844 PA2845 PA2846 PA2847 PA2848 ohrR
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Northern blot (ECO:0005653) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor not specified