A beta-gal assay was used to confirm expression, followed by a northern blot. A site-directed mutagenesis, followed by qRT-PCR, was used to show the importance of cysteine residues for expression. EMSA and DNAse footprinting were used to confirm binding of OhrR.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|