Curation Information

Publication
The SOS response of Listeria monocytogenes is involved in stress resistance and mutagenesis.;van der Veen S, van Schalkwijk S, Molenaar D, de Vos WM, Abee T, Wells-Bennik MH;Microbiology (Reading, England) 2010 Feb; 156(Pt 2):374-84 [19892760]
TF
LexA [Q8Y7H7, view regulon]
Reported TF sp.
Listeria monocytogenes EGD-e
Reported site sp.
Listeria monocytogenes EGD-e
Created by
Erill Lab
Curation notes
-
External databases
NCBI GEO (GSE12634) http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12634.

Experimental Process

DNA array for w-t and recA mutant after exposure to mit-C to identify target genes. Motif discovery on genes upregulated only in w-t background (i.e. RecA-dependent)

Transcription Factor Binding Sites


GTTGCGAACGTAGGTTCTGTG
AACACGAACACACTTTCTTTT
AAACAGAACATATGTTTTATC
AATAAGAACAAATGTTTGTAT
AATAAGAACGTATATTCGGTT
AATAAAAACATATGTTCGGTG
TTCAAGAACGTTTGTTCGTAT
AAAAAGAACGTATGTGCGAAA
AATGCGAAAATATGTTCGGTT
AATAAGAACATTTGTTCGTAT
TTTAAGAACGTTTGTTCGTAT
GTTGCGAACGTAGGTTCTGTG
AACACGAACACACTTTCTTTT
AAACAGAACATATGTTTTATC
AATAAGAACAAATGTTTGTAT
AATAAGAACGTATATTCGGTT
AATAAAAACATATGTTCGGTG
TTCAAGAACGTTTGTTCGTAT
AAAAAGAACGTATGTGCGAAA
AATGCGAAAATATGTTCGGTT
AATAAGAACATTTGTTCGTAT
TTTAAGAACGTTTGTTCGTAT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
GTTGCGAACGTAGGTTCTGTG lmo0157, lmo0158,
... ... lmo0157 lmo0158 lmo0156 lmo0155 lmo0154 lmo0153
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - repressor dimer
AACACGAACACACTTTCTTTT dnaE,
... ... dnaE lmo1575 lmo1576 lmo1577
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - repressor dimer
AAACAGAACATATGTTTTATC lmo1640, lmo1639, lmo1638,
... ... lmo1640 lmo1639 lmo1638 lmo1637 lmo1636 citB
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - repressor dimer
AATAAGAACAAATGTTTGTAT pcrA, ligA,
... ... pcrA ligA lmo1757 lmo1760
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - repressor dimer
AATAAGAACGTATATTCGGTT lmo2222, lmo2221, lmo2220,
... ... lmo2222 lmo2221 lmo2220 lmo2219 lmo2223
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - repressor dimer
AATAAAAACATATGTTCGGTG addB, lmo2267, lmo2266, lmo2265,
... ... addB lmo2267 lmo2266 lmo2265 lmo2269
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - repressor dimer
TTCAAGAACGTTTGTTCGTAT lmo2271,
... ... lmo2271 lmo2272
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - repressor dimer
AAAAAGAACGTATGTGCGAAA int,
... ... int lmo2331 lmo2330 lmo02333
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - repressor dimer
AATGCGAAAATATGTTCGGTT uvrB, uvrA,
... ... uvrB uvrA lmo2490 lmo2491 lmo2492
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - repressor dimer
AATAAGAACATTTGTTCGTAT lmo2675, lmo2676,
... ... lmo2675 lmo2676 lmo2674 lmo2673 lmo2677 lmo2678 lmo2679 kdpC kdpB kdpA
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - repressor dimer
TTTAAGAACGTTTGTTCGTAT lmo2828,
... ... lmo2828 lmo2829
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - repressor dimer