Curation Information

Publication
Metal ion regulation of gene expression. Fur repressor-operator interaction at the promoter region of the aerobactin system of pColV-K30.;de Lorenzo V, Giovannini F, Herrero M, Neilands JB;Journal of molecular biology 1988 Oct 20; 203(4):875-84 [3062182]
TF
Fur [P0A9A9, view regulon]
Reported TF sp.
ColV-K30
Reported site sp.
ColV-K30
Created by
Urooq Aqeel
Curation notes
Sites belong to plasmid ColV-K30, which is not available in NCBI.

Experimental Process

Site directed mutagenesis and DNAse I nicking were used to find the fur binding site. Expression of fur was experimentally tested by hydroxyl radical foot-printing and methylation protection experiments.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type