Curation Information

The Zur of Xanthomonas campestris functions as a repressor and an activator of putative zinc homeostasis genes via recognizing two distinct sequences within its target promoters.;Huang DL, Tang DJ, Liao Q, Li HC, Chen Q, He YQ, Feng JX, Jiang BL, Lu GT, Chen B, Tang JL;Nucleic acids research 2008 Aug; 36(13):4295-309 [18586823]
Zur [Q8PAL3, view regulon]
Reported TF sp.
Xanthomonas campestris pv. campestris str. 8004
Reported site sp.
Xanthomonas campestris pv. campestris str. 8004
Created by
Dinara Sagitova
Curation notes

Experimental Process

Genome-wide DNA microarray hybridization was done to identify Zur-regulated genes. gusA transcriptional fusion assays were performed to confirm Zur-mediated expression of these genes. EMSAs were done to confirm that Zur directly regulates its target genes. To further clarify whether Zur directly represses the transcription, in vitro transcription assays were performed. DNaseI footprinting analysis was employed to locate the Zur-binding sites within the promoter regions.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... XC_0267 XC_0266
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details In-vitro transcription (ECO:0001204) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details RACE PCR (ECO:0005661) - repressor not specified