Microarray studies were done to identify PerR-regulated genes. qPR_PCR analysis were then performed to validate microarray data. Presence of Per boxes in the identified genes was validated by scanning the M-type 3 strain MGAS315 genome. EMSAs confirmed that PerR bound directly to its target operons.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|