Comparative genomics with motif discovery (SignalX) and PSSM search to predict sites. Genome-wide expression profiling on a Zur mutant to identify candidate regulated sites. Expression also verified by RT-PCR and promoters mapped by RACE-PCR. EMSA and site-directed mutagenesis were performed to verify direct interaction of Zur with the putative Zur-binding sites .
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|