A series of EMSA's was performed to identify the LexA binding region. DNAse footprinting was used to show binding. Site directed mutagenesis, followed by EMSA, was also used to show binding. A PSSM search later performed further confirmed these results.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|