Curation Information

LexA-binding sequences in Gram-positive and cyanobacteria are closely related.;Mazón G, Lucena JM, Campoy S, Fernández de Henestrosa AR, Candau P, Barbé J;Molecular genetics and genomics : MGG 2004 Feb; 271(1):40-9 [14652736]
LexA [Q8YMM5, view regulon]
Reported TF sp.
Nostoc sp. PCC 7120
Reported site sp.
Nostoc sp. PCC 7120
Created by
Kathryn Cronise
Curation notes

Experimental Process

A series of EMSA's was performed to identify the LexA binding region. DNAse footprinting was used to show binding. Site directed mutagenesis, followed by EMSA, was also used to show binding. A PSSM search later performed further confirmed these results.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... alr4908 alr4907
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor dimer