A PSSM search was used with known phhA sites and the consensus sequence to identify potential binding sites. A beta-gal assay was performed to show that PhhR up-regulates the genes. EMSA and DNase footprinting was used to prove binding and ITC (isothermal titration calorimetry) was performed to show that binding is favorable.
Quantitative data format: Kd from quantitative EMSA.GTTTTGTAAAAATTATCAATCGATGA 1.71
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|