Proteome analysis identified a 3-fold derepression of ycgT expression in fur mutant cells compared to the wild-type cells. Inspection of the ycgT promoter region identified a putative Fur-binding site. Binding of Fur to the target promoter was shown by EMSAs. ycgT::lacZ transcriptional fusion confirmed that ycgT expression is repressed by Fur.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|