Curation Information

Role of the Fur regulon in iron transport in Bacillus subtilis.;Ollinger J, Song KB, Antelmann H, Hecker M, Helmann JD;Journal of bacteriology 2006 May; 188(10):3664-73 [16672620]
Fur [P54574, view regulon]
Reported TF sp.
Bacillus subtilis subsp. subtilis str. 168
Reported site sp.
Bacillus subtilis subsp. subtilis str. 168
Created by
Dinara Sagitova
Curation notes

Experimental Process

Proteome analysis identified a 3-fold derepression of ycgT expression in fur mutant cells compared to the wild-type cells. Inspection of the ycgT promoter region identified a putative Fur-binding site. Binding of Fur to the target promoter was shown by EMSAs. ycgT::lacZ transcriptional fusion confirmed that ycgT expression is repressed by Fur.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... ycgT ycgS nasF
Experimental technique details 2D PAGE (ECO:0005614) - Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Visual sequence inspection (nan) - repressor not specified