Curation Information

The H2O2 stress-responsive regulator PerR positively regulates srfA expression in Bacillus subtilis.;Hayashi K, Ohsawa T, Kobayashi K, Ogasawara N, Ogura M;Journal of bacteriology 2005 Oct; 187(19):6659-67 [16166527]
PerR [P71086, view regulon]
Reported TF sp.
Bacillus subtilis subsp. subtilis str. 168
Reported site sp.
Bacillus subtilis subsp. subtilis str. 168
Created by
Dinara Sagitova
Curation notes

Experimental Process

Binding of PerR protein to the srfA promoter region was confirmed by EMSA. Positive regulation of srfA expression by PerR was confirmed by using β-Galactosidase assays. The sequence bound by PerR in the srfA promoter was characterized by DNase I footprinting analysis.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... srfAB hxlR srfAA comS
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - activator dimer