A beta-gal assay was used to show that PhhR activates phhA expression. A PSSM search was used with the motifs of IHF (another NtrC TF) to identify a proximal and distal site. A beta-gal assay was used to determine that the distal site is necessary for phhA expression. EMSA and DNAse footprinting were used to show binding.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|