A beta-gal assay was used to show that PhhR activates phhA expression. A PSSM search was used with IHF (another NtrC TF) motifs to identify a proximal and distal site. A beta-gal assay was used with site directed mutagenesis to show that the proximal site is necessary for phhA activation. EMSA followed by DNAse footprinting was used to show binding.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|