Curation Information

Publication
RsaL provides quorum sensing homeostasis and functions as a global regulator of gene expression in Pseudomonas aeruginosa.;Rampioni G, Schuster M, Greenberg EP, Bertani I, Grasso M, Venturi V, Zennaro E, Leoni L;Molecular microbiology 2007 Dec; 66(6):1557-65 [18045385]
TF
RsaL [G3XD78, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Kathryn Cronise
Curation notes
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Experimental Process

A DNA microarray was performed to compare the expression profiles in the presence and absence of RsaL. EMSA was performed on 5 of the genes showing repression and 3 of these genes showed binding (phzA1, phzM, and hcnA).

Transcription Factor Binding Sites


TATGCAATCCACATC
TAGCAATCCCGCATA
TACCCACCTGTCATG
TATGCAATCCACATC
TAGCAATCCCGCATA
TACCCACCTGTCATG

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TATGCAATCCACATC phzA1,
... ... phzA1 phzM opmD mexI mexH mexG phzB1 phzC1 phzD1 phzE1 phzF1 phzG1
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - repressor dimer
TAGCAATCCCGCATA phzM,
... ... phzM opmD mexI mexH mexG phzA1 phzB1 phzC1 phzD1 phzE1 phzF1 phzG1
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - repressor dimer
TACCCACCTGTCATG hcnA,
... ... hcnA PA2192 hcnB hcnC
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - repressor dimer