Curation Information

Pseudomonas aeruginosa AlgR represses the Rhl quorum-sensing system in a biofilm-specific manner.;Morici LA, Carterson AJ, Wagner VE, Frisk A, Schurr JR, Höner zu Bentrup K, Hassett DJ, Iglewski BH, Sauer K, Schurr MJ;Journal of bacteriology 2007 Nov; 189(21):7752-64 [17766417]
AlgR [P26275, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Kathryn Cronise
Curation notes

Experimental Process

A microarray analysis identified rhlA as a gene that is differentially regulated by AlgR. A beta-gal assay confirmed that AlgR represses rhlA. EMSA was used to show binding. Mutagenesis followed by EMSA was used to show that one of the two putative sites is required for binding.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... rhlA PA3480 PA3481
Experimental technique details Beta-gal reporter assay - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor not specified