Curation Information

Detailed analysis of Helicobacter pylori Fur-regulated promoters reveals a Fur box core sequence and novel Fur-regulated genes.;Pich OQ, Carpenter BM, Gilbreath JJ, Merrell DS;Molecular microbiology 2012 Jun; 84(5):921-41 [22507395]
Fur [B5Z6G7, view regulon]
Reported TF sp.
Helicobacter pylori G27
Reported site sp.
Helicobacter pylori G27
Created by
Dinara Sagitova
Curation notes

Experimental Process

Putative Fur binding sites were identified by genome-wide search using Genamics Expression Software. Specific binding of Fur to the Fur boxes was shown via EMSA. Fur-regulated expression was confirmed by using cat reporter system. Additionally, introduction of a Fur box in the promoter region of a gene otherwise non-Fur-regulated cause this gene to exhibit Fur-dependent regulation.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... cagB cagA
Experimental technique details EMSA (ECO:0001807) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details PSSM site search (ECO:0005659) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator dimer