Fur binding-sites were identified and verified by employing the following techniques: 1.weight matrix (derived from known Fur binding sites in other bacteria) was used to carry out a genome-wide search of Fur binding sites in A.ferrooxidans. 2. FURTA was used to locate and isolate Fur binding sites 3. computationally predicted and FURTA isolated binding sites were evaluated for in vitro binding using EMSA. 4. the effect of Fur binding on transcription expression was evaluated by in vitro transcription
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|