A beta-gal assay was performed to show that Vfr activates rhlR expression. A consensus search identified putative binding sites. Each site was then knocked-out and a beta-gal assay was performed to see the effect. Three binding sites involved in rhlR activation were identified.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|