A beta-gal assay showed that Vfr is involved in rhlR expression. A consensus search identified 4 binding sites. Each site was knocked out and a beta-gal assay was performed to see the effect. One repression site was identified.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|