Curation Information

Iron-dependent transcription of the frpB gene of Helicobacter pylori is controlled by the Fur repressor protein.;Delany I, Pacheco AB, Spohn G, Rappuoli R, Scarlato V;Journal of bacteriology 2001 Aug; 183(16):4932-7 [11466300]
Fur [P0A9A9, view regulon]
Reported TF sp.
Helicobacter pylori G27
Reported site sp.
Helicobacter pylori G27
Created by
Urooq Aqeel
Curation notes

Experimental Process

First a DNase I footprint analysis showed that Fur binds specifically to a high-affinity site, then correct replacement of the wild-type sequence with the antibiotic resistance cassette was verified by means of PCR. The total RNA from the fur mutant strain was isolated, and transcription from the promoters under study was investigated by primer extension assays. Consensus sequence was also performed.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... HPG27_830 HPG27_829 ruvC
Experimental technique details Beta-gal reporter assay - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Primer Extension assay (ECO:0005657) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor dimer