Curation Information

Cloning and characterization of an Mn-containing superoxide dismutase (SodA) of Bordetella pertussis.;Graeff-Wohlleben H, Killat S, Banemann A, Guiso N, Gross R;Journal of bacteriology 1997 Apr; 179(7):2194-201 [9079904]
Fur [P0A3E5, view regulon]
Reported TF sp.
Bordetella pertussis CS
Reported site sp.
Bordetella pertussis CS
Created by
Urooq Aqeel
Curation notes

Experimental Process

Iron regulation demonstrated with beta-gal, primer extension and observation of enzyme activity. Fur site identified by visual inspection. To find the binding site, the researcher first manually located the fur box then for binding confirmation, performed a Fur titration assay (FURTA) and primer extension assay. For regulation method, a northern blot was performed.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... sodA BPTD_0189 BPTD_0191
Experimental technique details Ad-hoc qualitative phenotype observation (ECO:0005674) - Experimental technique details Beta-gal reporter assay - Experimental technique details FURTA (ECO:0005633) - Experimental technique details Primer Extension assay (ECO:0005657) - activator dimer