Researchers used LacZ fusions (in E. Coli) to determine the regulatory impact of LuxR on the putative lux boxes. They then ran EMSA's with those that showed a change in regulation.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|