Used site-directed mutagenesis to recreate the LuxT putative lux box in the upstream region of the pGEM plasmid, regulating luciferase. Used western blot analysis to compare protein output of wt vs ∆luxT. ∆luxT strain was generated using a deletion. Binding of LuxT was determined first by EMSA, then narrowed down via DNase Footprinting
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|