Curation Information

Transcriptional regulatory cascade for elastase production in Vibrio vulnificus: LuxO activates luxT expression and LuxT represses smcR expression.;Roh JB, Lee MA, Lee HJ, Kim SM, Cho Y, Kim YJ, Seok YJ, Park SJ, Lee KH;The Journal of biological chemistry 2006 Nov 17; 281(46):34775-84 [16971386]
LuxT [Q7MFA2, view regulon]
Reported TF sp.
Vibrio vulnificus MO6-24/O
Reported site sp.
Vibrio vulnificus MO6-24/O
Created by
Elliot White
Curation notes
Multiple variations of the DNase protected site were tried, including several truncations; none returned the correct site. The further site mentioned in the paper has no binding data associated with it.

Experimental Process

Used site-directed mutagenesis to recreate the LuxT putative lux box in the upstream region of the pGEM plasmid, regulating luciferase. Used western blot analysis to compare protein output of wt vs ∆luxT. ∆luxT strain was generated using a deletion. Binding of LuxT was determined first by EMSA, then narrowed down via DNase Footprinting

Transcription Factor Binding Sites

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type