Curation Information

Fnr-, NarP- and NarL-dependent regulation of transcription initiation from the Haemophilus influenzae Rd napF (periplasmic nitrate reductase) promoter in Escherichia coli K-12.;Stewart V, Bledsoe PJ;Journal of bacteriology 2005 Oct; 187(20):6928-35 [16199562]
FNR [P0A9E5, view regulon]
Reported TF sp.
Escherichia coli str. K-12 substr. MG1655
Reported site sp.
Escherichia coli str. K-12 substr. MG1655
Created by
Sepideh Zahiri
Curation notes

Experimental Process

The researchers used DNase I footprinting experiment to confirm the binding Fnr to napF control regionpromoter sequence. By doing a Beta-gal assay able to measure the activities transcriptional regulation of the mutant gene. In vitro transcription assay used to quantify changes in transcriptional regulation of napF promoter by Fnr ..the napF control region was amplified from plasmid by PCR.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... napF yojO napD napA napG napH napB napC ccmA ccmB ccmC ccmD ccmE ccmF ccmG ccmH eco
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details In-vitro transcription (ECO:0001204) - Experimental technique details qPCR [quantitative real-time] (ECO:0005660) - activator dimer