Curation Information

Widespread distribution of a lexA-regulated DNA damage-inducible multiple gene cassette in the Proteobacteria phylum.;Abella M, Erill I, Jara M, Mazón G, Campoy S, Barbé J;Molecular microbiology 2004 Oct; 54(1):212-22 [15458417]
LexA [P59479, view regulon]
Reported TF sp.
Pseudomonas putida KT2440
Reported site sp.
Pseudomonas putida KT2440
Created by
Erill Lab
Curation notes

Experimental Process

DNA-damage induction validated with RT-PCR. Binding of LexA-2 shown with EMSA and several site mutations.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
GAGTACAAATGTGCT lexA-2, PP_3117, PP_3118, dnaE2,
... ... lexA-2 PP_3117 PP_3118 dnaE2 PP_3115 PP_3114 PP_3113
Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor dimer