Curation Information

Publication
A green nonsulfur bacterium, Dehalococcoides ethenogenes, with the LexA binding sequence found in gram-positive organisms.;Fernández de Henestrosa AR, Cuñé J, Erill I, Magnuson JK, Barbé J;Journal of bacteriology 2002 Nov; 184(21):6073-80 [12374844]
TF
LexA [Q3Z9S7, view regulon]
Reported TF sp.
Dehalococcoides ethenogenes 195
Reported site sp.
Dehalococcoides ethenogenes 195
Created by
Erill Lab
Curation notes
-

Experimental Process

Binding site upstream of lexA identified through EMSA with several fragments. Serial site-directed mutagenesis on lexA site with EMSA to ascertain binding site.

Transcription Factor Binding Sites


AGAACAAGTGTTCT
AGAACAAGTGTTCT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
AGAACAAGTGTTCT
... ... rpsT lexA DET1641 fmt
Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor dimer